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f e modified sgrna scaffold  (Addgene inc)


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    Structured Review

    Addgene inc f e modified sgrna scaffold
    F E Modified Sgrna Scaffold, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+e+modified+sgrna+scaffold/pCC_09+-+hU6-BsmBI-sgRNA(E%2BF)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE+(Plasmid+%23139094)/pm39948336-282-20-27
    Average 93 stars, based on 9 article reviews
    f e modified sgrna scaffold - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094) and concentrated the ligation products using isopropanol precipitation. .. We electroporated the concentrated ligation product with Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245 mm BioAssay plates (Corning 431111) with LB / agar 100 ug/ml Carbenicillin.

    Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers.
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094)118 and concentrated the ligationproductsusing isopropanolprecipitation.Weelectroporated the concentrated ligation productwith Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245mmBioAssay plates (Corning 431111) with LB / agar 100 ug/mlCarbenicillin.Usingdilutionplating,wemeasureda representation of 500 colonies per gRNA. .. The bacteria were harvested after 14 h of incubation at 37 °C, scrapedwith 5-10ml of LBmedia andprocessedwith a PlasmidPlusmaxi prep kit (Qiagen 12965).Weused 1maxi prep column per 0.25 g of bacteria.

    Clone Assay:

    Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094) and concentrated the ligation products using isopropanol precipitation. .. We electroporated the concentrated ligation product with Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245 mm BioAssay plates (Corning 431111) with LB / agar 100 ug/ml Carbenicillin.

    Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers.
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094)118 and concentrated the ligationproductsusing isopropanolprecipitation.Weelectroporated the concentrated ligation productwith Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245mmBioAssay plates (Corning 431111) with LB / agar 100 ug/mlCarbenicillin.Usingdilutionplating,wemeasureda representation of 500 colonies per gRNA. .. The bacteria were harvested after 14 h of incubation at 37 °C, scrapedwith 5-10ml of LBmedia andprocessedwith a PlasmidPlusmaxi prep kit (Qiagen 12965).Weused 1maxi prep column per 0.25 g of bacteria.

    Modification:

    Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094) and concentrated the ligation products using isopropanol precipitation. .. We electroporated the concentrated ligation product with Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245 mm BioAssay plates (Corning 431111) with LB / agar 100 ug/ml Carbenicillin.

    Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers.
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094)118 and concentrated the ligationproductsusing isopropanolprecipitation.Weelectroporated the concentrated ligation productwith Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245mmBioAssay plates (Corning 431111) with LB / agar 100 ug/mlCarbenicillin.Usingdilutionplating,wemeasureda representation of 500 colonies per gRNA. .. The bacteria were harvested after 14 h of incubation at 37 °C, scrapedwith 5-10ml of LBmedia andprocessedwith a PlasmidPlusmaxi prep kit (Qiagen 12965).Weused 1maxi prep column per 0.25 g of bacteria.

    Ligation:

    Article Title: Comprehensive dissection of cis- regulatory elements in a 2.8 Mb topologically associated domain in six human cancers
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094) and concentrated the ligation products using isopropanol precipitation. .. We electroporated the concentrated ligation product with Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245 mm BioAssay plates (Corning 431111) with LB / agar 100 ug/ml Carbenicillin.

    Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers.
    Article Snippet: The gRNAs were synthesized as a pool (Twist Biosciences). .. We amplified and cloned the Twist pool as previously described117 using Gibson assembly into an all-in-one KRAB-dCas9 vector with the F + E modified sgRNA scaffold (pCC_09, Addgene 139094)118 and concentrated the ligationproductsusing isopropanolprecipitation.Weelectroporated the concentrated ligation productwith Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245mmBioAssay plates (Corning 431111) with LB / agar 100 ug/mlCarbenicillin.Usingdilutionplating,wemeasureda representation of 500 colonies per gRNA. .. The bacteria were harvested after 14 h of incubation at 37 °C, scrapedwith 5-10ml of LBmedia andprocessedwith a PlasmidPlusmaxi prep kit (Qiagen 12965).Weused 1maxi prep column per 0.25 g of bacteria.

    other:

    Article Title: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers.
    Article Snippet: The bacteria were harvested after 14 h of incubation at 37 °C, scrapedwith 5-10ml of LBmedia andprocessedwith a PlasmidPlusmaxi prep kit (Qiagen 12965).Weused 1maxi prep column per 0.25 g of bacteria.



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    f e modified sgrna scaffold  (Addgene inc)
    93
    Addgene inc f e modified sgrna scaffold
    F E Modified Sgrna Scaffold, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+e+modified+sgrna+scaffold/pCC_09+-+hU6-BsmBI-sgRNA(E%2BF)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE+(Plasmid+%23139094)/pm39948336-282-20-27
    Average 93 stars, based on 1 article reviews
    f e modified sgrna scaffold - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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